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KMID : 1025520050470030363
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Journal of Animal Science and Technology
2005 Volume.47 No. 3 p.363 ~ p.370
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Effect of β-Mercaptoethanol Supplement during In Vitro Maturation on IVM, IVF and Glutathione Level in Porcine Oocytes
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Oh Shin-Ae
Kim C.K.
Chung Yung-Chai
Chang Y.M.
Park Myung-Geol
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Abstract
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Experiments were conducted to determine the effects of beta-mercaptoethanol (β-ME) supplements to the in vitro maturation (IVM) medium on in vitro fertilization (IVF) and intracellular glutathione (GSH) concentration. Porcine cumulus-intact oocytes were matured in TCM-199 medium containing porcine follicular fluid, sodium pyruvate, D-glucose, FBS, hormonal supplements, and β-ME (0, 25, 50 and 100μM) for 36 to 46h. After culture, cumulus-free matured oocytes were co-incubated with epididymal spermatozoa for 18h. There were no significant differences in the maturation rate among treatment groups. However, increases (P<0.05) in intracellular GSH concentration before and after fertilization were observed in 50μM β-ME supplements to the IVM medium. Also, increases (P<0.05) in male pronuclear formation after IVF were observed in same treatment group. In conclusion, supplementing β-ME into the IVM medium increased intracellular GSH concentrations and increased fertilization in vitro.
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KEYWORD
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β-Mercaptoethanol, Glutathione, IVM, IVF, Porcine oocyte
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